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KMID : 0613820100200091287
Journal of Life Science
2010 Volume.20 No. 9 p.1287 ~ p.1293
Spatial Autocorrelation Analysis of Carex humilis on Mt. Giri by RAPD
Lee Bok-Kyu

Lee Byeong-Ryong
Huh Man-Kyu
Abstract
The spatial distribution of alleles and geographical distances of a Carex humilis population on Mt. Giri in Korea were studied. A total of 102 DNA fragments (bands) were found among 107 plants. Among these 102 bands, 48 (47.1%) bands were polymorphic. In a simple variability of subpopulations by the percentage of polymorphic bands, distances I and V exhibited the lowest variation (16.7%). Distance ¥· showed the highest variation (22.6%). The total genetic diversity (H) was 0.076 across species. Class ¥· had the highest H (0.093), while class I had the lowest (0.063). Genetic similarity of individuals was found among subpopulations at up to a scale of 60 m distance, and this was partly due to a combination of alleles. Within the Mt. Giri population, a strong spatial structure was observed for RAPD markers, indicating a very low amount of migration among subpopulations and that the distribution of individual genotypes of a given population was clumped. The present study demonstrated that analysis of RAPD markers could be successfully used to study the spatial and genetic structures of C. humilis.
KEYWORD
Genetic similarity, Carex humilis, spatial autocorrelation
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